The best Side of attune flow cytometer
The best Side of attune flow cytometer
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Info-enriched characterization of mobile populations. Morphological facts from pictures can insert for the richness of flow cytometry details. As an example, the determine demonstrates an normally traditional apoptosis assay employing Annexin V and PI, incorporating mobile imaging to characterize cells in Each and every populace to expose morphologically distinct capabilities. These insights couldn't have been gained from multiplex staining on your own.
No matter whether you’re a beginner or a professional, take a look at our methods to advance your investigation and know-how in flow cytometry.
Blend acoustic concentrating know-how with superior-parameter analysis for adaptable spectral unmixing and standard workflows.
Explore new insights into your experimental details by visualizing populations applying combined fluorescence and morphology facts. Our computer software interface wizards enable downsampling, concatenation (merged files) and dimensionality equipment which might be developed in the software for an intuitive, streamlined info Assessment knowledge.
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Right here, a qualified person has gated singlets confidently. After assessing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of much more than 4% aggregates.
Pair your Attune flow cytometer having an autosampler for greater workflow effectiveness, and incorporate robotic integration for max throughput.
Constant impression excellent even at substantial throughput Acoustic focusing in addition to a significant-pace camera Blend to graphic these T cells persistently at small or superior flow costs. Simply change concentrate and camera configurations to meet experimental demands.
Early signs of this contamination experienced at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also lets you use morphological features to find exciting subpopulations that could not be evident from flow cytometry info by itself.
Maybe most of all, these situations comprise cells of Obviously different phenotypes which may lead to incorrect conclusions concerning double beneficial gatherings (specifically in scarce populations).
Seem waves allow specific mobile alignment, resulting in Remarkable facts high quality and ~10X speedier sample flow prices.
Application note: Automatic image Assessment minimizes consumer-to-person variability in flow cytometry gating procedures
Developed-in dimensionality reduction applications elevated resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify envisioned inhabitants resolution in lysed entire human blood. User can view events from Each individual cluster for visual confirmation of cell phenotypes.
No wash/no lyse leukocyte Investigation with imaging. Cells were obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Expert UX/UI enables people to train AI designs for expanded apps. (A) The “Teach Design” attribute presents annotation resources and genuine-time design education precision.
Gating on these prolonged parameters helps you to quickly and correctly identify populations of interest and ensure gating approach with little if any manual assessment. The Attune application delivers entry to point out-of-the-art equipment Studying analysis instruments in just an expertly created consumer interface, rendering it extremely simple to operate.
The Attune Xenith cytometer is usually a higher-parameter spectral analyzer, giving equally https://gunnerpbzvc.ampedpages.com/flow-cytometer-a-revolutionary-tool-for-cell-analysis-60696613 spectral unmixing and traditional flow capabilities for complete and adaptable mobile Examination.
Agilent Price Guarantee guarantees you no less than 10 years of instrument use through the day you purchase. Usually, We'll credit history you With all the residual value of the system toward an upgraded model.
Right here, we show that by gating procedures making use of picture-based quantitative parameters can distinguish interacting cells from coincident functions to far more accurately evaluate interacting cells.